Part:BBa_K737044
NanC_Comparator
The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42. It enables strong competition with the galk::GFP to interact with spot42 which shows great potential for constructing sRNA-mediated circuit. This device mean to test feasibility of N/P comparator,replacing Nitrate,Phosphate inputs by IPTG,aTc Small RNA Spot42 controls the GFP mRNA translation by blocking the RBS in galK.The galK and E0040 is fused together using ClaI site to avoid stop condon in standard scar.J23106 is a moderate promotor,and galK’s RBS is a moderate one.
Spot42 sRNA is under control of aTc inducible device,the buffer RNA NanC is under control of IPTG inducible device.
The pairing hybridization energy is -19.2 kcal/mol. 12 bases are involved in the pairing. Enzymatic assays of nanC-LacZ fusion could give as strong as 47-fold repression. Besides, since nanC’s binding site didn’t coincide with galK’s, Corresponding mutant in spot42 to construct an orthogonal ratio sensor
Experimental Data
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1439
Illegal NheI site found at 1462
Illegal NheI site found at 2977
Illegal NheI site found at 3000 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2640
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1261
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1228
Illegal BsaI.rc site found at 3789
| n/a | NanC_Comparator |